Pubblicazioni

Autori:

Piccirilli, Alessandra; Cherubini, Sabrina; Azzini, Anna Maria; Tacconelli, Evelina; Lo Cascio, Giuliana; Maccacaro, Laura; Bazaj, Alda; Naso, Laura; Amicosante, Gianfranco; Ltcf-Veneto Working Group, Null; Perilli, Mariagrazia

Titolo:

Whole-Genome Sequencing (WGS) of Carbapenem-Resistant K. pneumoniae Isolated in Long-Term Care Facilities in the Northern Italian Region

Anno:

2021

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

Elettronico

Referee:

No

Nome rivista:

MICROORGANISMS

ISSN Rivista:

2076-2607

N° Volume:

9

Numero o Fascicolo:

9

Intervallo pagine:

1-13

Parole chiave:

Klebsiella pneumoniae; WGS; β-lactamases

Breve descrizione dei contenuti:

K. pneumoniae (KPN) is one of the widest spread bacteria in which combined resistance to several antimicrobial groups is frequent. The most common β-lactamases found in K. pneumoniae are class A carbapenemases, both chromosomal-encoded (i.e., NMCA, IMI-1) and plasmid-encoded (i.e., GES-enzymes, IMI-2), VIM, IMP, NDM, OXA-48, and extended-spectrum β-lactamases (ESBLs) such as CTX-M enzymes. In the present study, a total of 68 carbapenem-resistant KPN were collected from twelve long-term care facilities (LTCFs) in the Northern Italian region. The whole-genome sequencing (WGS) of each KPN strain was determined using a MiSeq Illumina sequencing platform and analysed by a bacterial analysis pipeline (BAP) tool. The WGS analysis showed the prevalence of ST307, ST512, and ST37 as major lineages diffused among the twelve LTCFs. The other lineages found were: ST11, ST16, ST35, ST253, ST273, ST321, ST416, ST1519, ST2623, and ST3227. The blaKPC-2, blaKPC-3, blaKPC-9, blaSHV-11, blaSHV-28, blaCTX-M-15, blaOXA-1, blaOXA-9, blaOXA-23, qnrS1, qnrB19, qnrB66, aac(6')-Ib-cr, and fosA were the resistance genes widespread in most LTCFs. In this study, we demonstrated the spreading of thirteen KPN lineages among the LTCFs. Additionally, KPC carbapenemases are the most widespread β-lactamase.

Id prodotto:

122688

Handle IRIS:

11562/1049932

ultima modifica:

15 novembre 2022

Citazione bibliografica:

Piccirilli, Alessandra; Cherubini, Sabrina; Azzini, Anna Maria; Tacconelli, Evelina; Lo Cascio, Giuliana; Maccacaro, Laura; Bazaj, Alda; Naso, Laura; Amicosante, Gianfranco; Ltcf-Veneto Working Group, Null; Perilli, Mariagrazia, Whole-Genome Sequencing (WGS) of Carbapenem-Resistant K. pneumoniae Isolated in Long-Term Care Facilities in the Northern Italian Region «MICROORGANISMS» , vol. 9 , n. 9 , 2021 , pp. 1-13

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