Pubblicazioni

Autori:

Koncan, Raffaella; Parisato, Giuliana; Sakarikou, C; Stringari, Gianni; Fontana, C; Favuzzi, Vincenza; Ligozzi, Marco; LO CASCIO, Giuliana

Titolo:

Direct identification of major Gram-negative pathogens in respiratory specimens by respiFISH® HAP Gram (-) Panel, a beacon-based FISH methodology

Anno:

2015

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Referee:

Sì

Nome rivista:

European Journal of Clinical Microbiology and Infectious Diseases

ISSN Rivista:

0934-9723

N° Volume:

34

Numero o Fascicolo:

10

Intervallo pagine:

2097-2102

Parole chiave:

espiFISH® HAP Gram (-) Panel

Breve descrizione dei contenuti:

Rapid detection of microorganisms in respiratory specimens is of paramount importance to drive the proper antibiotic regimen to prevent complications and transmission of infections. In the present study, the respiFISH® HAP Gram (-) Panel (miacom diagnostics GmbH, Duesseldorf, Germany) for the etiological diagnosis of hospital-acquired pneumonia was compared with the traditional culture method for the detection of major Gram-negative pathogens in respiratory specimens. respiFISH® combined the classical fluorescence in situ hybridization (FISH) technology with fluorescence-labeled DNA molecular beacons as probes. From September 2011 to January 2012, 165 samples were analyzed: the sensitivity and specificity were 94.39 and 87.93 %, respectively. Only six pathogens (3.6 %) were not identified with respiFISH®, while seven specimens (3 %) provided false-positive results. This beacon-based identification shortens the time to result by at least one work day, providing species-level identification within half an hour. Considering the high sensitivity and specificity and the significant time saving, the introduction of bbFISH® assays could effectively complement traditional systems in microbiology laboratories.

Id prodotto:

88116

Handle IRIS:

11562/926735

ultima modifica:

15 novembre 2022

Citazione bibliografica:

Koncan, Raffaella; Parisato, Giuliana; Sakarikou, C; Stringari, Gianni; Fontana, C; Favuzzi, Vincenza; Ligozzi, Marco; LO CASCIO, Giuliana, Direct identification of major Gram-negative pathogens in respiratory specimens by respiFISH® HAP Gram (-) Panel, a beacon-based FISH methodology «European Journal of Clinical Microbiology and Infectious Diseases» , vol. 34 , n. 10 , 2015 , pp. 2097-2102

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