Publications

Authors:

Dai Prè, Elena; Busato, Alice; Mannucci, Silvia; Vurro, Federica; De Francesco, Francesco; Riccio, Valentina; Solito, Samantha; Biswas, Reetuparna; Bernardi, Paolo; Riccio, Michele; Sbarbati, Andrea

Title:

In vitro characterization of adipose stem cells non-enzymatically extracted from the thigh and abdomen

Year:

2020

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

Sì

Name of journal:

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN of journal:

1422-0067

N° Volume:

21

Number or Folder:

3081

Page numbers:

1-16

Keyword:

Rigenera® protocol; abdomen; adipose stem cells; adipose tissue; non-enzymatic method; thigh

Short description of contents:

Autologous fat grafting is a surgical technique in which adipose tissue is transferred from one area of the body to another, in order to reconstruct or regenerate damaged or injured tissues. Before reinjection, adipose tissue needs to be purified from blood and cellular debris to avoid inflammation and preserve the graft viability. To perform this purification, different enzymatic and mechanical methods can be used. In this study, we characterized in vitro the product of a closed automatic device based on mechanical disaggregation, named Rigenera®, focusing on two sites of adipose tissue harvesting. At first, we optimized the Rigenera® operating timing, demonstrating that 60 s of treatment allows a higher cellular yield, in terms of the cell number and growth rate. This result optimizes the mechanical disaggregation and it can increase the clinical efficiency of the final product. When comparing the extracted adipose samples from the thigh and abdomen, our results showed that the thigh provides a higher number of mesenchymal-like cells, with a faster replication rate and a higher ability to form colonies. We can conclude that by collecting adipose tissue from the thigh and treating it with the Rigenera® device for 60 s, it is possible to obtain the most efficient product.

Web page:

https://doi.org/10.3390/ijms21093081

Product ID:

114341

Handle IRIS:

11562/1017529

Last Modified:

November 15, 2022

Bibliographic citation:

Dai Prè, Elena; Busato, Alice; Mannucci, Silvia; Vurro, Federica; De Francesco, Francesco; Riccio, Valentina; Solito, Samantha; Biswas, Reetuparna; Bernardi, Paolo; Riccio, Michele; Sbarbati, Andrea, In vitro characterization of adipose stem cells non-enzymatically extracted from the thigh and abdomen «INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES» , vol. 21 , n. 3081 , 2020 , pp. 1-16

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