Pubblicazioni

Autori:

Quintero Sierra, Lindsey Alejandra; Biswas, Reetuparna; Conti, Anita; Busato, Alice; Ossanna, Riccardo; Zingaretti, Nicola; Parodi, Pier Camillo; Conti, Giamaica; Riccio, Michele; Sbarbati, Andrea; De Francesco, Francesco

Titolo:

Highly pluripotent adipose-derived stem cell-enriched nanofat: a novel translational system in stem cell therapy

Anno:

2023

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

Elettronico

Referee:

Sì

Nome rivista:

CELL TRANSPLANTATION

ISSN Rivista:

0963-6897

N° Volume:

32

Intervallo pagine:

1-16

Parole chiave:

adipose stem cells; mechanical disaggregation; nanofat; stem cell therapy; stromal vascular fraction; translational therapy

Breve descrizione dei contenuti:

Fat graft is widely used in plastic and reconstructive surgery. The size of the injectable product, the unpredictable fat resorption rates, and subsequent adverse effects make it tricky to inject untreated fat into the dermal layer. Mechanical emulsification of fat tissue, which Tonnard introduced, solves these problems, and the product obtained was called nanofat. Nanofat is widely used in clinical and aesthetic settings to treat facial compartments, hypertrophic and atrophic scars, wrinkle attenuation, skin rejuvenation, and alopecia. Several studies demonstrate that the tissue regeneration effects of nanofat are attributable to its rich content of adipose-derived stem cells. This study aimed to characterize Hy-Tissue Nanofat product by investigating morphology, cellular yield, adipose-derived stem cell (ASC) proliferation rate and clonogenic capability, immunophenotyping, and differential potential. The percentage of SEEA3 and CD105 expression was also analyzed to establish the presence of multilineage-differentiating stress-enduring (MUSE) cell. Our results showed that the Hy-Tissue Nanofat kit could isolate 3.74 × 104 ± 1.31 × 104 proliferative nucleated cells for milliliter of the treated fat. Nanofat-derived ASC can grow in colonies and show high differentiation capacity into adipocytes, osteocytes, and chondrocytes. Moreover, immunophenotyping analysis revealed the expression of MUSE cell antigen, making this nanofat enriched of pluripotent stem cell, increasing its potential in regenerative medicine. The unique characteristics of MUSE cells give a simple, feasible strategy for treating a variety of diseases.

Pagina Web:

https://doi.org/10.1177/09636897231175968

Id prodotto:

134054

Handle IRIS:

11562/1095070

ultima modifica:

23 giugno 2023

Citazione bibliografica:

Quintero Sierra, Lindsey Alejandra; Biswas, Reetuparna; Conti, Anita; Busato, Alice; Ossanna, Riccardo; Zingaretti, Nicola; Parodi, Pier Camillo; Conti, Giamaica; Riccio, Michele; Sbarbati, Andrea; De Francesco, Francesco, Highly pluripotent adipose-derived stem cell-enriched nanofat: a novel translational system in stem cell therapy «CELL TRANSPLANTATION» , vol. 32 , 2023 , pp. 1-16

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo