Pubblicazioni

Assembly and trafficking of human small conductance Ca2+ -activated K+ channel SK3 are governed by different molecular domains.  (2005)

Autori:
Roncarati, Renza; Decimo, Ilaria; Fumagalli, Guido Francesco
Titolo:
Assembly and trafficking of human small conductance Ca2+ -activated K+ channel SK3 are governed by different molecular domains.
Anno:
2005
Tipologia prodotto:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Lingua:
Inglese
Formato:
A Stampa
Referee:
Nome rivista:
MOLECULAR AND CELLULAR NEUROSCIENCES
ISSN Rivista:
1044-7431
N° Volume:
28
Numero o Fascicolo:
2
Intervallo pagine:
314-325
Parole chiave:
ACTIVATED POTASSIUM CHANNELS; CELL-SURFACE EXPRESSION; HIPPOCAMPAL-NEURONS; RAT-BRAIN; CURRENTS; AFTERHYPERPOLARIZATION
Breve descrizione dei contenuti:
Intracellular trafficking is an important event in the control of type and number of ion channels expressed on the cell surface. In this study, we have identified molecular domains involved in assembly and trafficking of the human small conductance Ca2+-activated K+ channel SK3. Deletion of the N-terminus, the C-terminus, or the calmodulin-binding domain (CaMBD) led to retention of SK3 channels in the endoplasmic reticulum. Presence of the CaMBD allowed trafficking to the Golgi complex, and sequences downstream were required for efficient transport to the plasma membrane, suggesting several steps in the control of SK3 forward trafficking. Co-immunoprecipitation studies demonstrated that SK3 subunits lacking the N-terminus, the CaMBD, or the distal C-terminus, but not the entire C-terminus, were able to oligomerize with wild-type SK3 subunits. Thus, these two C-terminal regions of SK3 seem to contribute to assembly and trafficking of channels whereas the N-terminus is necessary for trafficking but not sufficient for oligomerization.
Id prodotto:
24930
Handle IRIS:
11562/24930
depositato il:
16 marzo 2012
ultima modifica:
11 maggio 2021
Citazione bibliografica:
Roncarati, Renza; Decimo, Ilaria; Fumagalli, Guido Francesco, Assembly and trafficking of human small conductance Ca2+ -activated K+ channel SK3 are governed by different molecular domains. «MOLECULAR AND CELLULAR NEUROSCIENCES» , vol. 28 , n. 22005pp. 314-325

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