- Autori:
-
DALLE CARBONARE, Luca Giuseppe; Valenti, Maria Teresa; Zanatta, Mirko; Donatelli, Luca; LO CASCIO, Vincenzo
- Titolo:
-
Circulating mesenchymal stem cells with abnormal osteogenic differentiation in patients with osteoporosis.
- Anno:
-
2009
- Tipologia prodotto:
-
Articolo in Rivista
- Tipologia ANVUR:
- Articolo su rivista
- Lingua:
-
Inglese
- Formato:
-
A Stampa
- Referee:
-
Sì
- Nome rivista:
- Arthritis and Rheumatism
- ISSN Rivista:
- 0004-3591
- N° Volume:
-
60
- Numero o Fascicolo:
-
11
- Intervallo pagine:
-
3356-3365
- Parole chiave:
-
mesenchymal stem cells; osteoporosis; osteoblastic lineage
- Breve descrizione dei contenuti:
- Objective. While the role of osteoclasts in bone
loss has been well investigated, the involvement of
osteoblast-lineage cells has not been completely elucidated.
Several genes contribute to normal osteoblastic
differentiation from mesenchymal stem cells (MSCs),
but an understanding of their role in the pathogenesis of
osteoporosis is still lacking. The present study was
undertaken to evaluate a possible alteration of osteogenic
gene expression as a mechanism contributing to
bone loss.
Methods. We studied the osteogenic differentiation
process in MSCs obtained from the peripheral
blood of 31 patients with osteoporosis and 20 normal
donors. The cells were evaluated by colony-forming
unit–fibroblastic assay and cultured in osteogenic medium
to analyze the transcription factors runt-related
transcription factor 2 (RUNX-2) and Sp7 and the
bone-related genes COL1A1, SPARC, and SPP1 after 3,
8, and 15 days of differentiation. In addition, to determine
possible differences between the 2 groups in terms
of osteoclastic and osteoblastic activation, we quantified
the osteoprotegerin (OPG) and RANKL levels in the
supernatants of osteoblastic culture.
Results. CirculatingMSCswere increased in osteoporosis
patients compared with normal donors. In contrast,
gene expression analysis revealed downregulation
of RUNX2, Sp7, COL1A1, SPARC, and SPP1
in patients with osteoporosis, associated with a lower
OPG:RANKL ratio.
Conclusion. These results suggest that an alteration
of osteoblastic differentiation may contribute to
the pathogenesis of osteoporosis. The noninvasive approach
used in the present study could be proposed as a
useful tool for studying mesenchymal involvement in
bone diseases.
- Id prodotto:
-
52981
- Handle IRIS:
-
11562/338048
- depositato il:
-
29 febbraio 2012
- ultima modifica:
-
14 novembre 2022
- Citazione bibliografica:
-
DALLE CARBONARE, Luca Giuseppe; Valenti, Maria Teresa; Zanatta, Mirko; Donatelli, Luca; LO CASCIO, Vincenzo,
Circulating mesenchymal stem cells with abnormal osteogenic differentiation in patients with osteoporosis.
«Arthritis and Rheumatism»
, vol.
60
, n.
11
,
2009
,
pp. 3356-3365
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