Pubblicazioni

Autori:

Marcela Nogueira Rabelo, Alves; Gabriele, Zanchetti; Alberto, Piccinotti; Silvia, Tameni; Bruno Spinosa De, Martinis; Polettini, ALDO ELIANO

Titolo:

Determination of cocaine and metabolites in hair by column-switching LC-MS-MS analysis

Anno:

2013

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

A Stampa

Referee:

Sì

Nome rivista:

Analytical and Bioanalytical Chemistry

ISSN Rivista:

1618-2642

N° Volume:

405

Numero o Fascicolo:

19

Editore:

Springer Verlag

Intervallo pagine:

6299-6306

Parole chiave:

cocaine; hair; analysis; column switching; liquid chromatography; mass spectrometry

Breve descrizione dei contenuti:

A method for rapid, selective, and robust determination of cocaine (CO) and metabolites in 5-mg hair samples was developed and fully validated using a column-switching liquid chromatography-tandem mass spectrometry system (LC-MS-MS). Hair samples were decontaminated, segmented, incubated overnight in diluted HCl, and centrifuged, and the diluted (1:10 with distilled water) extracts were analyzed in positive ionization mode monitoring two reactions per analyte. Quantifier transitions were: m/z 304.2→182.2 for CO, m/z 290.1→168.1 for benzoylecgonine (BE), and m/z 318.2→196.2 for cocaethylene (CE). The lower limit of quantification (LLOQ) was set at 0.05 ng/mg for CO and CE, and 0.012 ng/mg for BE. Imprecision and inaccuracy at LLOQ were lower than 20 % for all analytes. Linearity ranged between 0.05 and 50.0 ng/mg for CO and CE and 0.012 and 12.50 ng/mg for BE. Selectivity, matrix effect, process efficiency, recovery, carryover, cross talk, and autosampler stability were also evaluated during validation. Eighteen real hair samples and five samples from a commercial proficiency testing program were comparatively examined with the proposed multidimensional chromatography coupled with tandem mass spectrometry procedure and our reference gas chromatography coupled to mass spectrometry (GC-MS) method. Compared with our reference GC-MS method, column-switching technique and the high sensitivity of the tandem mass spectrometry detection system allowed to significantly reduce sample amount (×10) with increased sensitivity (×2) and sample throughput (×4), to simplify sample preparation, and to avoid that interfering compounds and ions impaired the ionization and detection of the analytes and deteriorate the performance of the ion source.

Id prodotto:

85523

Handle IRIS:

11562/881390

depositato il:

30 gennaio 2015

ultima modifica:

2 dicembre 2022

Citazione bibliografica:

Marcela Nogueira Rabelo, Alves; Gabriele, Zanchetti; Alberto, Piccinotti; Silvia, Tameni; Bruno Spinosa De, Martinis; Polettini, ALDO ELIANO, Determination of cocaine and metabolites in hair by column-switching LC-MS-MS analysis «Analytical and Bioanalytical Chemistry» , vol. 405 , n. 19 , 2013 , pp. 6299-6306

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