Publications

Authors:

Cavallini, Sara; Unali, Ilaria; Bertoncelli, Anna; Cecchetto, Riccardo; Mazzariol, Annarita

Title:

Ceftazidime/avibactam resistance is associated with different mechanisms in KPC-producing Klebsiella pneumoniae strains

Year:

2021

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

No

Name of journal:

ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA

ISSN of journal:

1217-8950

N° Volume:

68

Number or Folder:

4

Page numbers:

235-239

Keyword:

KPC mutations; OmpK35-Ompk36 mutations; ceftazidime-avibactam resistance

Short description of contents:

This study focused on Klebsiella pneumoniae isolates that were resistant or had low susceptibility to a combination of ceftazidime/avibactam. We aimed to investigate the mechanisms underlying this resistance. A total of 24 multi-drug resistant isolates of K. pneumoniae were included in the study. The phenotypic determination of carbapenemase presence was based on the CARBA NP test. NG-Test CARBA 5 was also performed, and it showed KPC production in 22 out 24 strains. The molecular characterisation of blaKPC carbapenemase gene, ESBL genes (blaCTX-M, blaTEM, and blaSHV) and porin genes ompK35/36 was performed using the PCR. Finally, ILLUMINA sequencing was performed to determine the presence of genetic mutations.Various types of mutations in the KPC sequence, leading to ceftazidime/avibactam resistance, were detected in the analysed resistant strains. We observed that KPC-31 harboured the D179Y mutation, the deletion of the amino acids 167-168, and the mutation of T243M associated with ceftazidime/avibactam resistance. The isolates that did not present carbapenemase alterations were found to have other mechanisms such as mutations in the porins. The mutations both on the KPC-3 enzyme and in the porins confirmed, that diverse mechanisms confer resistance to ceftazidime/avibactam in K. pneumoniae.

Product ID:

123186

Handle IRIS:

11562/1051919

Last Modified:

February 23, 2023

Bibliographic citation:

Cavallini, Sara; Unali, Ilaria; Bertoncelli, Anna; Cecchetto, Riccardo; Mazzariol, Annarita, Ceftazidime/avibactam resistance is associated with different mechanisms in KPC-producing Klebsiella pneumoniae strains «ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA» , vol. 68 , n. 4 , 2021 , pp. 235-239

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