Publications

Authors:

Grisotto, Jacopo; Tavakolian Haghighi, Shima; Sasan, Atena Poor; Pedron, Serena; Brunelli, Matteo; Moretti, Ugo; Paolone, Giovanna

Title:

Neurotoxic effects of rotenone and deltamethrin prolonged exposure on adult zebrafish

Year:

2025

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

No

Name of journal:

SCIENTIFIC REPORTS

ISSN of journal:

2045-2322

N° Volume:

15

Number or Folder:

1

Page numbers:

1-14

Keyword:

Animals; Apoptosis; Behavior, Animal; Brain; Dopaminergic Neurons; Insecticides; Neurotoxicity Syndromes; Nitriles; Pyrethrins; Rotenone; Water Pollutants, Chemical; Zebrafish

Short description of contents:

The presence of pesticides in aquatic ecosystems has become an increasing concern. Contamination of ground and surface water results from substances escaping wastewater treatment plants (WWTP) and leaching from soil. Pesticides pose a significant threat to the aquatic environment, as even trace concentrations can damage the central nervous systems (CNS) of animals and humans. Rotenone (ROT), an electron transport chain inhibitor, causes selective dopaminergic (DA) degeneration, while deltamethrin (DM), a widely used type II pyrethroid insecticide, is known for its neurotoxic effect. We assessed the impact of chronic exposure to ROT (2 mu g/L, 4 weeks) and DM (1 and 2.5 mu g/L, 15 days) on the central nervous system of adult zebrafish. TUNEL assay analysis (n = 15 in total) revealed both pesticides triggered cell death in different brain regions of fish, including areas involved in sensory, motor, and cognitive processes. DM additionally affected regions associated with complex behaviors such as learning, memory, and decision-making. Immunohistological analyses (n = 12 in total) showed loss of DA neurons in the areas involved in the motor control of animals exposed to both pesticides. These neurotoxic effects were further supported by behavioral changes (n = 38 in total) in the Novel Tank Test (NTT), indicating alterations in movement and anxiety-like behavior. Our findings confirm that chronic sub-threshold exposure to chemicals present in environmental waters causes significant damage to cerebral tissue, leading to apoptosis and behavioral alterations.

Product ID:

146934

Handle IRIS:

11562/1168548

Last Modified:

August 21, 2025

Bibliographic citation:

Grisotto, Jacopo; Tavakolian Haghighi, Shima; Sasan, Atena Poor; Pedron, Serena; Brunelli, Matteo; Moretti, Ugo; Paolone, Giovanna, Neurotoxic effects of rotenone and deltamethrin prolonged exposure on adult zebrafish «SCIENTIFIC REPORTS» , vol. 15 , n. 1 , 2025 , pp. 1-14

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