Publications

Authors:

Moore, Ps; Barbi, Stefano; Donadelli, Massimo; Costanzo, Chiara; Bassi, Claudio; Palmieri, Marta; Scarpa, Aldo

Title:

Gene expression profiling after treatment with the histone deacetylase inhibitor trichostatin A reveals altered expression of both pro- and anti-apoptotic genes in pancreatic adenocarcinoma cells.

Year:

2004

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

Biochimica et Biophysica Acta (BBA)

ISSN of journal:

0006-3002

N° Volume:

1693

Number or Folder:

3

:

Elsevier

Page numbers:

167-176

Keyword:

Trichostatin A; Pancreatic cancer; Expression profiling

Short description of contents:

The histone deacetylase inhibitor trichostatin A (TSA) has been previously shown to block cellular growth in G2 and induce apoptosis in human pancreatic cancer cell lines. In order to better understand this phenomenon, we have analyzed the gene expression profiles in PaCa44 cells after treatment with TSA using microarrays containing 22,283 probesets. TSA was found to cause both the induction and repression of a large number of genes, although the number whose expression was up-regulated was greater than the number of genes that were downregulated. When a threshold value of 3 was used as a cutoff level, a total of 306 (3.4%) of the detectable genes had altered expression. When categorized according to cellular function, the differentially expressed genes were found to be involved in a wide variety of cellular processes, including cell proliferation, signaling, regulation of transcription, and apoptosis. Moreover, Sp1/Sp3 transcription factor binding sites were significantly more abundant among TSA-induced genes. One prominent feature was the increased ratio between the levels of expression of pro-apoptotic (BIM) and anti-apoptotic (Bcl-XL and Bcl-W) genes. This result was confirmed in eight additional pancreatic cancer cell lines after treatment with TSA, suggesting that this event may be a strong determinant for the induction of apoptosis by TSA.

Web page:

http://preview.ncbi.nlm.nih.gov/pubmed/15363630

Product ID:

24241

Handle IRIS:

11562/24241

Deposited On:

June 22, 2012

Last Modified:

November 7, 2022

Bibliographic citation:

Moore, Ps; Barbi, Stefano; Donadelli, Massimo; Costanzo, Chiara; Bassi, Claudio; Palmieri, Marta; Scarpa, Aldo, Gene expression profiling after treatment with the histone deacetylase inhibitor trichostatin A reveals altered expression of both pro- and anti-apoptotic genes in pancreatic adenocarcinoma cells. «Biochimica et Biophysica Acta (BBA)» , vol. 1693 , n. 3 , 2004 , pp. 167-176

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