Publications

Authors:

Poli, Albino; Guglielmini, Elena; Sembeni, Silvia; Spiazzi, Massimiliano; Dellaglio, Franco; Rossi, Franca; Torriani, Sandra

Title:

Detection of Staphylococcus aureus and enterotoxin genotype diversity in Monte Veronese, a Protected Designation of Origin (PDO) Italian cheese.

Year:

2007

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Referee:

Sì

Name of journal:

Letters Applied Microbiology

ISSN of journal:

0266-8254

N° Volume:

45

Number or Folder:

5

:

blackwell synergy

Page numbers:

529-534

Keyword:

Food safety, Multiplex PCR, PDO cheese, Real-time PCR detection, SE genes diffusion, Staphylococcus aureus

Short description of contents:

Aims: To evaluate the risk associated with the load and enterotoxigenicity of Staphylococcus aureus in Monte Veronese, a PDO (Protected Designation of Origin) cheese of the Lessinia area (Verona, Italy). Methods and Results: Staphylococcus aureus was quantified by a conventional culture method and by a nucA targeted real-time PCR assay developed in this study. Staphylococcus aureus numbers in cheese were higher than the limit tolerated by the Italian food legislation in 78% instances, according to both detection methods. Multiplex PCR tests for 17 Staph. aureus enterotoxin (SE) genes were applied to nucleic acids extracted from curds, cheeses and Staph. aureus isolates. The SE gene diversity appeared reduced after ripening. The gene encoding SED was found most frequently in dairy samples and the enterotoxin genes ser, sed, seg and sem predominated in the isolates. Conclusions: The occurrence of enterotoxigenic Staph. aureus strains with complex SE genotypes in this PDO cheese at numbers often exceeding the Italian tolerance threshold represents an important risk factor. Significance and Impact of the Study: The high frequency of contamination of Monte Veronese PDO cheese and, expectedly, similar typical productions from raw milk, by enterotoxigenic Staph. aureus imposes a tighter hygienic control in the earlier manufacturing phases.

Product ID:

36990

Handle IRIS:

11562/307782

Deposited On:

July 6, 2012

Last Modified:

December 1, 2022

Bibliographic citation:

Poli, Albino; Guglielmini, Elena; Sembeni, Silvia; Spiazzi, Massimiliano; Dellaglio, Franco; Rossi, Franca; Torriani, Sandra, Detection of Staphylococcus aureus and enterotoxin genotype diversity in Monte Veronese, a Protected Designation of Origin (PDO) Italian cheese. «Letters Applied Microbiology» , vol. 45 , n. 5 , 2007 , pp. 529-534

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