- Authors:
-
Formaggio, Elena; Cantù, Cinzia; Chiamulera, Cristiano; Fumagalli, Guido Francesco
- Title:
-
p75 neurotrophin receptor distribution and transport in cultured neurons.
- Year:
-
2008
- Type of item:
-
Articolo in Rivista
- Tipologia ANVUR:
- Articolo su rivista
- Language:
-
Inglese
- Format:
-
A Stampa
- Referee:
-
Sì
- Name of journal:
- Neuroscience Research
- ISSN of journal:
- 0168-0102
- N° Volume:
-
62
- Number or Folder:
-
1
- Page numbers:
-
32-42
- Keyword:
-
p75; neurotrophin receptors; transport; GFP
- Short description of contents:
- In this work, we define a GFP-tagged version of the p75 neurotrophin receptor (p75GFP) as a useful molecular tool for studying its distribution and cellular dynamics. Expression and subcellular localization of p75GFP have been characterized in non-neuronal (HEK 293) and in neuronal (cortical and hippocampal) cells. By monitoring movements of intracellular p75GFP in living cultured hippocampal neurons, we found that the chimeric protein was transported by tubulo-vesicular structures both anterogradely (0.1-0.5mum/s) and retrogradely (0.1-1.1mum/s), with a faster component in retrogradely moving structures. Movements of the p75GFP-containing structures were inhibited by treatment with the microtubule disrupting agent nocodazole. Our data indicate that p75GFP is a reliable tool for studying spatial and cellular properties of p75 in CNS neurons and that p75 transport inside neurons is mediated by microtubule-associated motors.
- Product ID:
-
45973
- Handle IRIS:
-
11562/319747
- Deposited On:
-
August 25, 2008
- Last Modified:
-
November 27, 2022
- Bibliographic citation:
-
Consulta la scheda completa presente nel
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