Publications

Authors:

Torriani, Sandra; Gatto, Veronica; Sembeni, Silvia; Tofalo, R.; Suzzi, G.; Belletti, N.; Gardini, F.; BOVER CID, S.

Title:

Rapid detection and quantification of tyrosine decarboxylasegene (tdc) and its expression in Gram-Positive bacteriaassociated with fermented foods using PCR-based methods

Year:

2008

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

Journal of Food Protection

ISSN of journal:

0362-028X

N° Volume:

71

Number or Folder:

1

Page numbers:

93-101

Keyword:

tyrosine decarboxylase gene; fermented food; PCR-based methods

Short description of contents:

In this study, PCR-based procedures were developed to detect the occurrence and quantify the expression of the tyrosine decarboxylase gene (tdc) in gram-positive bacteria associated with fermented foods. Consensus primers were used in conventional and reverse transcription PCR to analyze a collection of 87 pure cultures of lactic acid bacteria and staphylococci. All enterococci, Staphylococcus epidermidis, Lactobacillus brevis, Lactobacillus curvatus, and Lactobacillus fermentum strains and 1 of 10 Staphylococcus xylosus strains produced amplification products with the primers DEC5 and DEC3 in accordance with results of the screening plate method and with previously reported result obtained with high-performance liquid chromatography. No amplicons were obtained for tyramine-negative strains, confirming the high specificity of these new primers. A novel quantitative real-time PCR assay was successfully applied to quantify tdc and its transcript in pure cultures and in meat and meat products. This assay allowed estimation of the influence of different variables (pH, temperature, and NaCl concentration) on the tdc expression of the tyraminogenic strain Enterococcus faecalis EF37 after 72 h of growth in M17 medium. Data obtained suggest that stressful conditions could induce greater tyrosine decarboxylase activity. The culture-independent PCR procedures developed here may be used for reliable and fast detection and quantification of bacterial tyraminogenic activity without the limitations of conventional techniques.

Product ID:

55087

Handle IRIS:

11562/323865

Deposited On:

July 6, 2012

Last Modified:

November 18, 2022

Bibliographic citation:

Torriani, Sandra; Gatto, Veronica; Sembeni, Silvia; Tofalo, R.; Suzzi, G.; Belletti, N.; Gardini, F.; BOVER CID, S., Rapid detection and quantification of tyrosine decarboxylasegene (tdc) and its expression in Gram-Positive bacteriaassociated with fermented foods using PCR-based methods «Journal of Food Protection» , vol. 71 , n. 1 , 2008 , pp. 93-101

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo