Publications

Authors:

LO CASCIO, Giuliana; Balzaj, A; Maccacaro, L; Fontana, Roberta; Centonze, Anna Rita; Sorrentino, A; Mazzariol, Annarita

Title:

Performance of different commercial methods for determining minimum inhibitory concentrations of glycopeptides and linezolid against blood isolates of Staphylococcus aureus

Year:

2013

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Referee:

Sì

Name of journal:

JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE

ISSN of journal:

2213-7165

N° Volume:

1

Number or Folder:

3

Page numbers:

163-169

Keyword:

Vancomycin; Teicoplanin; VITEK 2; Sensititre; Etest

Short description of contents:

The aim of this study was to determine the accuracy of commercial systems (VITEK (R) 2, Etest and Sensititre (R)) in determining the minimum inhibitory concentrations of vancomycin, teicoplanin and linezolid of Staphylococcus aureus strains and to evaluate the reproducibility of each system in a clinical microbiology laboratory. In total, 115 strains of S. aureus isolated from blood cultures were tested with all three commercial methods as well as the broth microdilution method, which is designated as the standard for glycopeptides and linezolid. Fourteen different S. aureus strains were included in a reproducibility test for all methods and antibiotics. For these strains, antimicrobial susceptibility testing was repeated 10 times on different days with all four methods, each time using the same inoculum. All three commercial methods exhibited similar performance in categorisation of nearly all of the meticillin-susceptible S. aureus (MSSA) isolates. Discrepancies were registered for meticillin-resistant S. aureus (MRSA); 2.5\% of the strains in the intermediate or resistant category with the VITEK 2 system were not recognised as resistant by Etest and Sensititre. Moreover, none of the three commercial methods provided accurate results compared with homemade broth microdilution. Reproducibility of vancomycin and teicoplanin was 100\% with VITEK 2 and Sensititre and 98.75\% with Etest. Microdilution showed a reproducibility of 95.6\% with vancomycin and 83.1\% with teicoplanin. In contrast to previous reports, the best agreement with microdilution was exhibited by VITEK 2 both for MSSA and MRSA. For the antibiotics tested, the best reproducibility was obtained with the VITEK 2 and Sensititre systems. (C) 2013 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

Product ID:

89623

Handle IRIS:

11562/932621

Last Modified:

December 1, 2022

Bibliographic citation:

LO CASCIO, Giuliana; Balzaj, A; Maccacaro, L; Fontana, Roberta; Centonze, Anna Rita; Sorrentino, A; Mazzariol, Annarita, Performance of different commercial methods for determining minimum inhibitory concentrations of glycopeptides and linezolid against blood isolates of Staphylococcus aureus «JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE» , vol. 1 , n. 3 , 2013 , pp. 163-169

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