Publications

Authors:

Bria, Emilio; Pilotto, Sara; Simbolo, Michele; Fassan, Matteo; DE MANZONI, Giovanni; Carbognin, L; Sperduti, I; Brunelli, Matteo; Cataldo, Ivana; Tomezzoli, Anna; Mafficini, Andrea; Turri, G; Karachaliou, N; Rosell, R; Tortora, Giampaolo; Scarpa, Aldo

Title:

Comprehensive molecular portrait using next generation sequencing of resected intestinal-type gastric cancer patients dichotomized according to prognosis

Year:

2016

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

Sì

Name of journal:

SCIENTIFIC REPORTS

ISSN of journal:

2045-2322

N° Volume:

6

Page numbers:

1-8

Keyword:

LIGHT-EMITTING-DIODES; INTERNAL QUANTUM EFFICIENCY; VAPOR-PHASE EPITAXY; WELL-STRUCTURE; GAN; LAYERS; LASERS; PHOTOLUMINESCENCE; PHOTONICS; ALLOYS

Short description of contents:

In this study, we evaluated whether the presence of genetic alterations detected by next generation sequencing may define outcome in a prognostically-selected and histology-restricted population of resected gastric cancer (RGC). Intestinal type RGC samples from 34 patients, including 21 best and 13 worst prognostic performers, were studied. Mutations in 50 cancer-associated genes were evaluated. A significant difference between good and poor prognosis was found according to clinico-pathologic factors. The most commonly mutated genes in the whole population were PIK3CA (29.4%), KRAS (26.5%), TP53 (26.5%) MET (8.8%), SMAD4 (8.8%) and STK11 (8.8%). Multiple gene mutations were found in 14/21 (67%) patients with good prognosis, and 3/13 (23%) in the poor prognosis group. A single gene alteration was found in 5/21 (24%) good and 6/13 (46%) poor prognosis patients. No mutation was found in 2/21 (9.5%) and 4/13 (31%) of these groups, respectively. In the overall series, ß-catenin expression was the highest (82.4%), followed by E-Cadherin (76.5%) and FHIT (52.9%). The good prognosis group was characterized by a high mutation rate and microsatellite instability. Our proof-of-principle study demonstrates the feasibility of a molecular profiling approach with the aim to identify potentially druggable pathways and drive the development of customized therapies for RGC.

Note:

The page range is based on PDF file available on the journal website

Product ID:

91502

Handle IRIS:

11562/938556

Last Modified:

November 15, 2022

Bibliographic citation:

Bria, Emilio; Pilotto, Sara; Simbolo, Michele; Fassan, Matteo; DE MANZONI, Giovanni; Carbognin, L; Sperduti, I; Brunelli, Matteo; Cataldo, Ivana; Tomezzoli, Anna; Mafficini, Andrea; Turri, G; Karachaliou, N; Rosell, R; Tortora, Giampaolo; Scarpa, Aldo, Comprehensive molecular portrait using next generation sequencing of resected intestinal-type gastric cancer patients dichotomized according to prognosis «SCIENTIFIC REPORTS» , vol. 6 , 2016 , pp. 1-8

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