Pubblicazioni

Autori:

Zanotti, R; Ambrosetti, Achille; Lestani, M; Ghia, P; Pattaro, C; Remo, Andrea; Zanetti, F; Stella, S; Perbellini, O; Prato, G; Guida, G; CALIGARIS CAPPIO, F; Menestrina, Fabio; Pizzolo, Giovanni; Chilosi, Marco

Titolo:

ZAP-70 expression, as detected by immunohistochemistry on bone marrow biopsies from early-phase CLL patients, is a strong adverse prognostic factor.

Anno:

2007

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Referee:

No

Nome rivista:

Leukemia

ISSN Rivista:

0887-6924

N° Volume:

21

Intervallo pagine:

102-109

Parole chiave:

chronic lymphocytic leukaemia, ZAP-70 expression,chronic lymphocytic leukaemia, ZAP-70 expression,

Breve descrizione dei contenuti:

Zeta-associated protein-70 (ZAP-70), mostly assessed by flowcytometry (FC), recently emerged as reliable prognostic factor in chronic lymphocytic leukaemia (CLL) at presentation. We evaluated ZAP-70 expression in 156 CLL patients by immunohistochemistry (IHC) on formalin-fixed bone marrow (BM) biopsies at diagnosis. At presentation, 117 patients (75%) were with Binet stage A, 27 (17%) stage B and 12 (8%) stage C. Median follow-up was 61 months (range 6–242). ZAP-70 was expressed in neoplastic lymphocytes of 69 patients (44%). Concordance between ZAP-70 by IHC and ZAP-70 by FC, immunoglobulin heavy chain variable genes (IGHV) mutational status and CD38 expression was found in 41/46 (89%), 41/49 (80%) and in 60/88 (68%) tested cases, respectively. ZAP-70 expression significantly correlated with advanced Binet stage (B–C), diffuse BM infiltration, increased lactate dehydrogenase (LDH) and b2-microglobulin serum levels and lymphocyte doubling time o12 months. ZAP-70 positivity was significantly related to poorer time to progression (median 16 months vs 158 of ZAP-70-negative cases) (Po0.0001) and overall survival (median 106 months vs not reached) (P¼0.0002); this correlation was confirmed at multivariate analysis. ZAP-70 expression correlated with poorer outcome also when evaluated only in the 117 stage A patients. In conclusion, immunohistological detection of ZAP-70 on formalin-fixed BM biopsies at diagnosis appears a useful methodological approach to identify patients with poor prognosis in CLL.

Id prodotto:

34695

Handle IRIS:

11562/233489

depositato il:

18 febbraio 2008

ultima modifica:

15 novembre 2022

Citazione bibliografica:

Zanotti, R; Ambrosetti, Achille; Lestani, M; Ghia, P; Pattaro, C; Remo, Andrea; Zanetti, F; Stella, S; Perbellini, O; Prato, G; Guida, G; CALIGARIS CAPPIO, F; Menestrina, Fabio; Pizzolo, Giovanni; Chilosi, Marco, ZAP-70 expression, as detected by immunohistochemistry on bone marrow biopsies from early-phase CLL patients, is a strong adverse prognostic factor. «Leukemia» , vol. 21 , 2007 , pp. 102-109

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