Publications

Authors:

Bifari, Francesco; Decimo, Ilaria; Chiamulera, Cristiano; Bersan, Emanuela; Malpeli, Giorgio; Johansson, Jan Evert; Lisi, Veronica; Bonetti, Bruno; Fumagalli, Guido Francesco; Pizzolo, Giovanni; Krampera, Mauro

Title:

Novel stem/progenitor cells with neuronal differentiation potential reside in the leptomeningeal niche

Year:

2009

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

ISSN of journal:

1582-1838

N° Volume:

13

Number or Folder:

9B

Page numbers:

3195-3208

Keyword:

neural stem cells; leptomeninges; stem cell niche; neuronal differentiation

Short description of contents:

Abstract Stem cells capable of generating neural differentiated cells are recognized by the expression of nestin and reside in specific regions of the brain, namely hippocampus, subventricular zone (SVZ), and olfactory bulb. For other brain structures, such as leptomeninges, which contribute to the correct cortex development and functions, there is no evidence so far that they may contain stem/precursor cells. In this work, we show for the first time that nestin-positive cells are present in rat leptomeninges during development up to adulthood. The newly identified nestin-positive cells can be extracted and expanded in vitro both as neurospheres, displaying high similarity with SVZ-derived neural stem cells, and as homogeneous cell population with stem cell features. In vitro expanded stem cell population can differentiate with high efficiency into excitable cells with neuronal phenotype and morphology. Once injected into adult brain, these cells survive and differentiate into neurons, thus showing that their neuronal differentiation potential is operational also in vivo. In conclusion, our data provide evidence that a specific population of immature cells endowed of neuronal differentiation potential is resident in the leptomeninges throughout the life. As leptomemniges cover the entire central nervous system, these findings could have relevant implications for studies on cortical development and for regenerative medicine applied to neurological disorders.

Product ID:

48840

Handle IRIS:

11562/327452

Deposited On:

January 20, 2010

Last Modified:

November 16, 2022

Bibliographic citation:

Bifari, Francesco; Decimo, Ilaria; Chiamulera, Cristiano; Bersan, Emanuela; Malpeli, Giorgio; Johansson, Jan Evert; Lisi, Veronica; Bonetti, Bruno; Fumagalli, Guido Francesco; Pizzolo, Giovanni; Krampera, Mauro, Novel stem/progenitor cells with neuronal differentiation potential reside in the leptomeningeal niche «JOURNAL OF CELLULAR AND MOLECULAR MEDICINE» , vol. 13 , n. 9B , 2009 , pp. 3195-3208

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo